Health Center

1. PLoS ONE: Enriched Monolayer Precursor Cell Cultures from Micro ...
... with or without 0.5 µM retinoic acid (Sigma), or with 0.5% fetal bovine serum and human Brain Derived Neurotrophic Factor (BDNF; 100 ng/ml). Recombinant human Insulin (Sigma) was used at a concentration of 20 µg/ml. ...

2. Insulin Growth Factor 1 Receptor/PI3K/AKT Survival Pathway in ...
Preparation of Osmotically Intact Rod Outer Segment Discs Osmotically intact ROS discs were prepared by Ficoll flotation.21 ROS was prepared from frozen bovine retinas according to the method described.22 The ROS pellet was resuspended ...

3. (IUCr) Crystallization of proteins on functionalized surfaces
Bovine pancreatic insulin (EC No. 234-291-2), ribonuclease A from bovine pancreas (type I-A; EC 3.1.27.5), polystyrene, N-propyltriethoxysilane, 3-aminopropyltriethoxysilane and Sigmacote (chlorinated organopolysiloxane in heptane) were ...

4. PLoS ONE: A Combination of Nutriments Improves Mitochondrial ...
In the present study, we investigated the effects of a combination of nutrients on insulin resistance and mitochondrial biogenesis/function in skeletal muscle of type 2 diabetic Goto–Kakizaki rats. ...

5. A BIOENGINEERED HUMAN SKIN EQUIVALENT (HSE) FOR THE EVALUATION OF ...
pituitary extract (0.2% v/v), bovine insulin (5 μg/ml),. hydrocortisone (0.18 μg/ml), bovine transferrin (5 μg/ml),. and human epidermal growth factor (0.2 ng/ml). Keratinocytes passages 2 or 3 were used for the. preparation of the skin ...

6. Stimulation of Corneal Epithelial Migration by a Synthetic Peptide ...
They were passaged in supplemented hormonal epithelial medium (SHEM), composed of DMEM-F12 supplemented with 15% heat-inactivated FBS, bovine insulin (5 µg/mL), cholera toxin (0.1 µg/mL), rhEGF (10 ng/mL), and gentamicin (40 µg/mL). ...

7. (IUCr) On the routine use of soft X-rays in macromolecular ...
Zn-free bovine pancreatic insulin (Sigma, Lot No. 033K1449) crystals were prepared by mixing protein dissolved at a concentration of 18 mg ml-1 in 50 mM Na2HPO4 pH 10.4 and 1 mM ethylenediaminetetraacetic acid (EDTA) and reservoir ...

8. Breast Cancer Research | Full text | Mammary carcinoma behavior is ...
... 2× P+S, 2% bovine serum albumin fraction V [Invitrogen], 5 mg/ml Insulin [Sigma, St Louis, MO, USA], 0.5 mg/ml hydrocortisone [Sigma], 10 ng/ml cholera toxin [Sigma], and 3 mg/ml Collagenase [Worthington Biochemical Corp., Lakewood, ...

9. Arthritis Research & Therapy | Full text | Abnormal insulin-like ...
Cells were then exposed to 50 ng/ml insulin-like growth factor (IGF)-1 for 5 minutes. The top panel shows a representative western blot of pPKB and total PKB levels in one normal and one OA osteoblast preparation. ...

10. Breast Cancer Research | Full text | Mouse mammary tumors display ...
HC11 cells were maintained in growth medium (RPMI 1640 medium augmented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 5 μg/ml insulin (Sigma, Saint Louis, MO, USA) and 2 mM glutamine (Hyclone). ...